Structural determinats of cobrotoxin affect its mobility on sodium dodecyl sulfate polyacrylamide gel
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چکیده
منابع مشابه
High-temperature sodium dodecyl sulfate polyacrylamide gel electrophoresis.
The run time for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) can be reduced to as little as 5 min by running vertical slab gels at 70°C. High-temperature SDS-PAGE increases the migration rate of protein bands several fold and is as effective as cooling in preventing uneven migration rates across the width of the gel. Additional benefits of hightemperature SDS-PAGE inclu...
متن کاملNonspecific interactions alter lipopolysaccharide patterns and protein mobility on sodium dodecyl sulfate polyacrylamide gels.
In testing whether bacterial lipopolysaccharide (LPS) was a natural substrate for an esterase from the soil amebae Dictyostelium discoideum, we observed altered banding patterns of the LPS and changed protein mobility on sodium dodecyl sulfate (SDS) polyacrylamide gels after incubation of LPS with the enzyme. The initial interpretation of these results was that the enzyme had removed ester-link...
متن کاملAnalysis of the subunit structure of protochlorophyllide holochrome by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
The subunit structures of protochlorophyllide holochrome (PCH) and chlorophyllide holochrome (CH) were studied by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. PCH from leaves of dark-grown (Phaseolus vulgaris var. red kidney) is a polymeric pigment-protein complex of approximately 600,000 daltons. It is composed of 12 to 14 polypeptides of 45,000 daltons, when examined prior...
متن کاملRenaturation of enzymes after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.
A number of enzymes, including amylases, dehydrogenases, and proteases, were shown to be renaturable after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Enzyme activity was detected in situ by action on substrates introduced into the gel and subsequent staining of either the product or unreacted substrate. This technique combines the advantage of enzyme identific...
متن کاملSimplified sodium dodecyl sulfate-polyacrylamide gel electrophoresis of unconcentrated urine with enhanced resolution and detection sensitivity.
We applied a simple sodium dodecyl sulfate-polyacrylamide gel electrophoresis method to urine. The method, developed for serum protein analysis (Clin Chem 1984;30:475-9), has a high sample throughput and gives excellent resolution with unconcentrated urine. It clearly distinguishes and characterizes proteinuric urine (7.5 microL) by Coomassie Blue staining and gives complex silver-stained patte...
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ژورنال
عنوان ژورنال: IUBMB Life
سال: 1996
ISSN: 1521-6543
DOI: 10.1080/15216549600201873